By Donald B. McCormick, Lemuel D. Wright
The severely acclaimed laboratory average, Methods in Enzymology, is likely one of the such a lot hugely revered guides within the box of biochemistry. for the reason that 1955, every one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate today—truly a necessary book for researchers in all fields of lifestyles sciences
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Additional info for Vitamins and Coenzymes Part D
Samples prepared with this solution should be diluted with cold 50 mM perchloric acid prior to analysis. Discussion The retention times are such that assay of ascorbic acid can be performed at a sampling rate of 12-20 per hour depending on the chromatographic packing material chosen. Multivitamin formulations do not present any particular problems for iodimetric titration methods or LCEC except in those preparations containing ferrous sulfate. For these sample types, the chromatographic approach is superior.
This effect was due to the plug of high ionic strength that passed through the column. Samples prepared with the extracting solution were diluted prior to injection on the anion-exchange column. Cold 50 mM perchloric acid was found to be the ideal medium to inhibit the oxidation of ascorbic acid. Samples and standards were frequently diluted with this solution and stored refrigerated for several hours. Approximately 99% of the ascorbic acid remained after 12 hr when prepared in cold dilute perchloric acid.
E. Strothkamp and C. R. Dawson, Biochemistry 16, 1926 (1977). K. G. Krul and C. R. Dawson, Bioinorg. Chem. 7, 71 (1977). METHODS IN ENZYMOLOGY, VOL. 62 Copyright © 1979 by Academic Press, Inc. All rights of reproduction in any form reserved, ISBN 0-12-181962-0  ASCORBATE OXIDASE 31 activity has been defined as the amount of enzyme that causes an initial rate of oxygen uptake of 10/A/min under the prescribed conditions. Specific activity is expressed in units per milligram of protein, or, alternatively, as units per microgram of copper.