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By H. N. Munro

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I t seems to indicate that one should not rely on cytochrome c comparisons as a phylogenetic measurement in vertebrates. Less inconsistency is evident when the comparison is made over a range of phyla. 116 0 . 26 MBDC: 51 2 4 . 59 two yeasts, and a mold. A steady trend is evident as the phylogenetic difference increases. This is seen in the MBDC averages at the foot of the table, which express the difference between each organism and the other eight. 1. Rhodospirillum rubrum Cytochrome c2 An analysis of the sequence of Rhodospirillum rubrum cytochrome c2 was published by Dus and Sletten (1968).

One thousand seems a large number, but it may be within the bounds of possibility, because 180 replacements have been found in a chains of various species and 103 replacements in ß chains. Only 17 species of animals have been examined, and in some cases, only the a chain was analyzed. In the light of these findings at the evolutionary level, it is evident that many changes in hemoglobin are possible. It therefore seems likely that there could be 1000 different mutational vari­ ants in human hemoglobin A.

Each tRNA molecule contains a "loop" of seven unpaired bases, the middle three of which are the anticodon. , A pairs with U, and C with G. The pairing between the first base of the anticodon and the third base of the codon is less specific (Crick, 1966), and it appears to be as follows: U G C I in in in in the the the the anticodon anticodon anticodon anticodon pairs pairs pairs pairs with with with with G or C U or C G U,C, or A in in in in the the the the codon ; codon ; codon ; codon. 36 THOMAS H.

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