By R. W. Fairbrother (Auth.)
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Spore-bearing bacteria are more resistant to heat than the vegetative forms. By heating a suspension at 80° C. for 10 minutes vegetative bacteria are destroyed while the spores are unaffected. On subsequent cultivation the spores germinate into vegetative forms. This method is used largely for the isolation of anaerobic spore-bearing organisms from contaminated material. 7. Animal Inoculation. In some cases the most satisfactory method of isolating bacteria is by the inoculation of susceptible laboratory animals, such as mice or guinea-pigs.
Of each dilution is placed in a Petri dish, melted agar, cooled to 50°-55° C, is added and the contents of the plate mixed by gentle agitation. After incubation at the optimum temperature for 24-48 hours, the total number of colonies on plates containing a countable number is obtained and an average made after the dilutions have been adjusted. c. can be estimated with a moderate degree of accuracy. 2. Total Count. B. 49 £ 50 A TEXT-BOOK OF BACTERIOLOGY may be estimated by various methods, but none is particularly accurate.
C. of each dilution is placed in a Petri dish, melted agar, cooled to 50°-55° C, is added and the contents of the plate mixed by gentle agitation. After incubation at the optimum temperature for 24-48 hours, the total number of colonies on plates containing a countable number is obtained and an average made after the dilutions have been adjusted. c. can be estimated with a moderate degree of accuracy. 2. Total Count. B. 49 £ 50 A TEXT-BOOK OF BACTERIOLOGY may be estimated by various methods, but none is particularly accurate.